董悦生

个人信息Personal Information

副教授

博士生导师

硕士生导师

性别:男

毕业院校:中国协和医科大学

学位:博士

所在单位:生物工程学院

学科:生物化工. 微生物学. 微生物与生化药学

办公地点:辽宁省大连市高新园区凌工路2号大连理工大学西部校区生物工程学院309室

联系方式:辽宁省大连市高新园区凌工路2号大连理工大学生物工程学院

电子邮箱:yshdong@dlut.edu.cn

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虎杖发酵液中白藜芦醇的分离纯化工艺研究

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发表时间:2022-10-06

发表刊物:中草药

期号:2

页面范围:223-227

ISSN号:0253-2670

摘要:Objective To isolate and purify resveratrol fromermentation broth of Polygonum cuspi-datum. Methods Macroporous resin and polyamide column chromatography were combined to purify res- veratrol from the extract in fermentation broth of P. cuspidatum, and the adsorption dynamics of resvera-trol was evaluated. Results Apparent static adsorptive capacities of resveratrol on macroporous resin and polyamide were 11.70 and 57.9 mg/g, respectively, and apparent dynamic adsorptive capacities were 12.47 and 58.98 mg/g,respectively. Both of the adsorption isotherms correlate well with Langmuir type. After macroporous resin and polyamide two-step column chromatography, the purity of resveratro] reached 95.8%, with the yield of 69.8%. Conclusion It is proved the method is potential for industrial applica-tion clue to its simple procedure, lower cost as well as high purity and yield of resveratrol.

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