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Indexed by:期刊论文

Date of Publication:2013-03-01

Journal:COLLOIDS AND SURFACES B-BIOINTERFACES

Included Journals:SCIE、EI、PubMed

Volume:103

Page Number:38-44

ISSN No.:0927-7765

Key Words:Immunosensor; Microcystin-LR; Graphene; Carbon nanosphere; Toxins

Abstract:A graphene-based immunosensor was fabricated for the detection of microcystin-LR using a horseradish peroxidase-carbon nanosphere-antibody system for signal amplification. Graphene and chitosan were used as immobilization materials to improve the electrochemical performance of the electrode. Signal amplification was achieved using multienzyme functionalized carbon nanosphere. This approach provided a linear range from 0.05 to 15 mu g L-1 microcystin-LR with a detection limit of 0.016 mu g L-1. Analytical detection of microcystin-LR in environmental water samples were demonstrated by comparing the results obtained using immunosensor and high-performance liquid chromatography. This electrochemical immunosensor showed good performance with high sensitivity, acceptable stability, repeatability and the potential for use in routine water quality monitoring for various toxins. (c) 2012 Elsevier B.V. All rights reserved.