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论文类型：期刊论文

发表时间：2014-06-01

发表刊物：SENSORS AND ACTUATORS B-CHEMICAL

收录刊物：SCIE、EI

卷号：196

页面范围：532-538

ISSN号：0925-4005

关键字：Photoelectrochemical immunoassay; Silicon naonowire; Graphene quantum dot; Microcystin-LR

摘要：A photoelectrochemical immuno sensor was developed by incorporating graphene quantum dots (GQDs) and highly oriented silicon nanowires (SiNWs) for the determination of microcystin-LR (MC-LR) in water samples. The GQDs/SiNWs heterostructure was employed for signal transduction and biocompatible nanoscaffold for antibody (Ab) immobilization. The results showed that the fabricated GQDs/SiNWs electrode exhibited better photoelectrochemical performance than that of SiNWs. The specific recognition of MC-LR affected the optoelectronic properties of the Ab/GQDs/SiNWs electrode, leading to the photocurrent decreases. In the linear range from 0.1 mu g L-1 to 10 mu g L-1, the photocurrent decrease was proportional to the concentration of microcystin-LR and the linear regression equation was Y = 59.67 - 6.08X with a limit of detection of 0.055 mu g L-1. The immunosensor possessed good sensitivity, selectivity and acceptable reusability for the determination of microcystin-LR, which was successfully applied in determining MC-LR in real water samples. (C) 2014 Elsevier B.V. All rights reserved.