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论文类型：期刊论文

发表时间：2019-03-05

发表刊物：ANALYTICAL CHEMISTRY

收录刊物：SCIE

卷号：91

期号：5

页面范围：3336-3341

ISSN号：0003-2700

关键字：Fluorescence; Mitochondria; Photons; Polycyclic aromatic hydrocarbons; Probes, Confocal scanning microscopy; Detection limits; Fluorescent probes; Minor grooves; Mitochondrial DNA (mtDNA); Non destructive; Structured illumination microscopy; Super resolution imaging, Nucleic acids

摘要：Many mitochondria-related diseases are associated with the mutation of mitochondrial DNA (mtDNA). Therefore, visualizing its dynamics in live cells is essential for the understanding of the function of mtDNA transcription and translation. By employing carbazole as the framework and designing a module for DNA minor-groove binding, here we have developed a novel fluorescent probe with a large Stokes shift (lambda(ab) = 480 nm and lambda(em) = 620 nm), CNQ, for mtDNA detection and visualization. It is almost nonfluorescent in PBS buffer and exhibits 182-fold enhancement in fluorescence within 20 s after the application of mtDNA in the solution, with a detection limit of 55.1 mu g/L. Using dual-color Hessian-structured illumination microscopy, we have demonstrated that CNQ-labeled mtDNA structures are distinct from those labeled by TFAM-EGFP. Finally, we have used two-photon confocal scanning microscopy (lambda(ex) = 850 nm) to monitor the nondestructive doxorubicin-induced mtDNA damage in live cells.