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个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：Director, State Key laboratory of Fine Chemicals
其他任职：精细化工国家重点实验室主任、国务院学科评议组成员
性别：男
毕业院校：大连理工大学
学位：博士
所在单位：化工学院
学科：应用化学. 精细化工. 化学生物学
办公地点：大连理工大学精细化工国家重点实验室
西部校区化工实验楼F-202#
http://peng-group.dlut.edu.cn/
联系方式：大连理工大学精细化工国家重点实验室 西部校区化工实验楼F-202 辽宁省大连市高新区凌工路2号，大连116024 Tel: 0411-84986306; Fax: 0411-84986292；课题组网址：http://peng-group.dlut.edu.cn/
电子邮箱：pengxj@dlut.edu.cn
Self-Assembly Trigger Signal Amplification for MicroRNA Sensing in Living Cells with GSH-Cleavable Nanoprobes
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论文类型：期刊论文
发表时间：2021-04-12
发表刊物：INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
卷号：59
期号：47
页面范围：20582-20590
ISSN号：0888-5885
摘要：Accurate sensing of endogenous microRNAs remains a great challenge for unsatisfactory signal-to-background ratios of traditional techniques. Meanwhile, low sensitivity and insufficient specificity limit the application of most detection methods. Herein, a newly designed GSH-cleavable nanoprobe with the capacity of signal amplification has been proposed for monitoring and imaging of low abundance intracellular miRNA both in a fluorescence assay and in living cells. In this work, endogenous glutathione (GSH) has been introduced to the construction of an miRNA recognizing system for realizing the cyclical process of target. The effective work of GSH makes the circulation of target miRNA possible through spontaneous biocleavage, leading to high detection efficiency and improved sensitivity of nanoprobes. Moreover, minimized false-positive signals and great decrement in environmental disturbance are obtained through ratiometric measurements. Besides, GSH-cleavable nanoprobes provide the enhanced fluorescence resonance energy transfer (FRET) signal between cancer cells and normal cells, which made the practical application in discrimination more reliable.