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个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：Director, State Key laboratory of Fine Chemicals
其他任职：精细化工国家重点实验室主任、国务院学科评议组成员
性别：男
毕业院校：大连理工大学
学位：博士
所在单位：化工学院
学科：应用化学. 精细化工. 化学生物学
办公地点：大连理工大学精细化工国家重点实验室
西部校区化工实验楼F-202#
http://peng-group.dlut.edu.cn/
联系方式：大连理工大学精细化工国家重点实验室 西部校区化工实验楼F-202 辽宁省大连市高新区凌工路2号，大连116024 Tel: 0411-84986306; Fax: 0411-84986292；课题组网址：http://peng-group.dlut.edu.cn/
电子邮箱：pengxj@dlut.edu.cn
Precise photodynamic therapy: Penetrating the nuclear envelope with photosensitive carbon dots
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论文类型：期刊论文
发表时间：2021-04-12
发表刊物：CARBON
卷号：159
页面范围：74-82
ISSN号：0008-6223
关键字：Precise photodynamic therapy; Carbon dots; RNA-Loading; Nucleus penetration; Precise treatment
摘要：Photodynamic therapy (PDT) has drawn significant attention as a noninvasive cancer treatment. At present, PDT applications are limited in vivo because of the short lifetime and limited diffusion distance of cytotoxic reactive oxygen species (ROS) in the cellular environment. We propose to overcome this challenge by generating limited and short-lived ROS that are localized in key organelles, particularly the nucleus, in tumor cells. We developed Se/N-doped carbon dots (Se/N-CDs) as a photosensitizer for PDT. The Se/N-CDs were shown to bind RNA selectively by digestion experiments, isothermal titration microcalorimetry, and cell dyeing with RNA probes. Se/N-CDs near the nuclear membrane produced ROS that damaged the membrane under light irradiation, permitting the entry of more Se/N-CDs and thereby improving the conversion efficiency of photosensitization. Furthermore, in vitro and in vivo experiments indicated that the Se/N-CDs could inhibit tumor growth more effectively by PDT within the nucleus. (C) 2019 Elsevier Ltd. All rights reserved.