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Indexed by:Journal Papers

Date of Publication:2019-03-05

Journal:ANALYTICAL CHEMISTRY

Included Journals:SCIE

Volume:91

Issue:5

Page Number:3336-3341

ISSN No.:0003-2700

Key Words:Fluorescence; Mitochondria; Photons; Polycyclic aromatic hydrocarbons; Probes, Confocal scanning microscopy; Detection limits; Fluorescent probes; Minor grooves; Mitochondrial DNA (mtDNA); Non destructive; Structured illumination microscopy; Super resolution imaging, Nucleic acids

Abstract:Many mitochondria-related diseases are associated with the mutation of mitochondrial DNA (mtDNA). Therefore, visualizing its dynamics in live cells is essential for the understanding of the function of mtDNA transcription and translation. By employing carbazole as the framework and designing a module for DNA minor-groove binding, here we have developed a novel fluorescent probe with a large Stokes shift (lambda(ab) = 480 nm and lambda(em) = 620 nm), CNQ, for mtDNA detection and visualization. It is almost nonfluorescent in PBS buffer and exhibits 182-fold enhancement in fluorescence within 20 s after the application of mtDNA in the solution, with a detection limit of 55.1 mu g/L. Using dual-color Hessian-structured illumination microscopy, we have demonstrated that CNQ-labeled mtDNA structures are distinct from those labeled by TFAM-EGFP. Finally, we have used two-photon confocal scanning microscopy (lambda(ex) = 850 nm) to monitor the nondestructive doxorubicin-induced mtDNA damage in live cells.