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HicAB toxin-antitoxin complex from Escherichia coli: expression and crystallization

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Indexed by:Journal Papers

First Author:Yang, Jingsi

Correspondence Author:Zhang, JJ (reprint author), Dalian Univ Technol, Coll Chem, 2 Linggong Rd, Dalian 116024, Peoples R China.; Liu, QS (reprint author), Chinese Acad Sci, Inst High Energy Phys, Multidiscipline Res Ctr, Yuquan Rd 19B, Beijing 100049, Peoples R China.

Co-authors:Xu, Bingshuang,Gao, Zengqiang,Zhou, Ke,Liu, Peng,Dong, Yuhui,Zhang, Jianjun,Liu, Quansheng

Date of Publication:2017-09-01

Journal:ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS

Included Journals:Scopus、SCIE、PubMed

Volume:73

Issue:Pt 9

Page Number:505-510

ISSN No.:2053-230X

Key Words:toxin-antitoxin systems; HicAB; persister cells; cell stress; Escherichia coli

Abstract:Toxin-antitoxin (TA) systems are widespread in both bacteria and archaea, where they enable cells to adapt to environmental cues. TA systems play crucial roles in various cellular processes, such as programmed cell death, cell growth, persistence and virulence. Here, two distinct forms of the type II toxin-antitoxin complex HicAB were identified and characterized in Escherichia coli K-12, and both were successfully overexpressed and purified. The two proposed forms, HicAB(L) and HicAB(S), differed in the presence or absence of a seven-amino-acid segment at the N-terminus in the antitoxin HicB. The short form HicABS readily crystallized under the conditions 0.1 M Tris-HCl pH 8.0, 20%(w/v) PEG 6000, 0.2 Mammonium sulfate. The HicAB(S) crystal diffracted and data were collected to 2.5 angstrom resolution. The crystal belonged to space group I222 or I2(1)2(1)2(1), with unit-cell parameters a = 67.04, b = 66.31, c = 120.78 angstrom. Matthews coefficient calculation suggested the presence of two molecules each of HicA and HicB(S) in the asymmetric unit, with a solvent content of 55.28% and a Matthews coefficient (V-M) of 2.75 angstrom 3 Da(-1).

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