李淑晶

个人信息Personal Information

副教授

硕士生导师

性别:女

毕业院校:大连理工大学

学位:博士

所在单位:生物工程学院

办公地点:生物楼416

电子邮箱:lsj@dlut.edu.cn

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Sumoylation of TCF21 downregulates the transcriptional activity of estrogen receptor-alpha

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论文类型:期刊论文

发表时间:2016-05-03

发表刊物:ONCOTARGET

收录刊物:SCIE、PubMed、Scopus

卷号:7

期号:18

页面范围:26220-26234

ISSN号:1949-2553

关键字:sumoylation; TCF21; ER alpha; transcriptional activity; proliferation

摘要:Aberrant estrogen receptor-alpha (ER alpha) signaling is recognized as a major contributor to the development of breast cancer. However, the molecular mechanism underlying the regulation of ER alpha in breast cancer is still inconclusive. In this study, we showed that the transcription factor 21 (TCF21) interacted with ER alpha, and repressed its transcriptional activity in a HDACs-dependent manner. We also showed that TCF21 could be sumoylated by the small ubiquitin-like modifier SUMO1, and this modification could be reversed by SENP1. Sumoylation of TCF21 occurred at lysine residue 24 (K24). Substitution of K24 with arginine resulted in complete abolishment of sumoylation. Sumoylation stabilized TCF21, but did not affect its subcellular localization. Sumoylation of TCF21 also enhanced its interaction with HDAC1/2 without affecting its interaction with ER alpha. Moreover, sumoylation of TCF21 promoted its repression of ERa transcriptional activity, and increased the recruitment of HDAC1/2 to the pS2 promoter. Consistent with these observations, sumoylation of TCF21 could inhibit the growth of ER alpha-positive breast cancer cells and decreased the proportion of S-phase cells in the cell cycle. These findings suggested that TCF21 might act as a negative regulator of ER alpha, and its sumoylation inhibited the transcriptional activity of ER alpha through promoting the recruitment of HDAC1/2.