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个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：Professor at the Institute of Advanced Measurement & Control Technology
其他任职：先进检测与控制技术研究所所长
性别：男
毕业院校：上海交通大学
学位：博士
所在单位：控制科学与工程学院
学科：控制理论与控制工程. 化学工程
办公地点：大连理工大学控制科学与工程学院先进检测与控制技术研究所
大连市凌工路2号大连理工大学海山楼A座724室
联系方式：Tel:(0411)84706465 实验室网站：http://act.dlut.edu.cn/
电子邮箱：tliu@dlut.edu.cn
Design of online off-gas analysis system for anaerobic ABE fermentation and the strategy for improving biobutanol production
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论文类型：期刊论文
发表时间：2016-05-01
发表刊物：PROCESS BIOCHEMISTRY
收录刊物：SCIE、EI、Scopus
卷号：51
期号：5
页面范围：555-560
ISSN号：1359-5113
关键字：Off-gas analysis; ABE fermentation; Neutral red; Fibrous bed bioreactor (FBB) system
摘要：Hydrogen and carbon dioxide as the main products of anaerobic acetone-butanol-ethanol (ABE) fermentation are very crucial for metabolic flux analysis. Up to the present, rare study has been devoted to real-time analysis on gas products of anaerobic fermentation systems. An on-line off-gas analysis system was therefore designed to monitor the off-gas produced in ABE fermentation with good accuracy. To deal with the influence from oxygen and off-gas variation during the fermentation, a strictly sealed monitoring system was set up to accurately analyze the off-gas while maintaining the oxygen-free environment in the bioreactor. At the meantime, the effect of electron carrier (neutral red) on metabolic distribution was studied in the free cell and immobilized cell fermentations. Compared to 13.7 g/L butanol (21.3 g/L ABE) and 20.8 L H-2 in 44 h in free cell fermentation, 16.8 g/L butanol (26.8 g/L ABE) and 23.9 L H-2 in 35 h in immobilized cell fermentation were produced in the addition of neutral red, suggesting that more NADH was produced for solvents biosynthesis. It is therefore demonstrated that the established on-line off-gas analysis system and optimization strategy for improving butanol production facilitate metabolic analysis and biofuels production in anaerobic ABE fermentation. (C) 2016 Elsevier Ltd. All rights reserved.