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论文类型：期刊论文

发表时间：2018-10-15

发表刊物：FRONTIERS IN CHEMISTRY

收录刊物：PubMed、SCIE

卷号：6

页面范围：488

ISSN号：2296-2646

关键字：fluorescence probe; formaldehyde; Daphnia magna; large Stokes shifts; Aza-Cope reaction; bioimaging

摘要：Formaldehyde (FA), a highly reactive carbonyl species, plays significant role in physiological and pathological functions. However, elevated FA will lead to cognitive impairments, memory loss and various neurodegenerative diseases due to its potent DNA and protein cross-linking mechanisms. In this work, a fluorescence probe, BD-CHO, based on benz-2-oxa-1, 3- diazole (BD) skeleton, was designed and synthesized for detection of FA via Aza-Cope reaction with high selectivity and large Stokes shifts (about 118 nm). BD-CHO was successfully applied to monitor the changes FA level in living cells, and kidney tissues of mice. Importantly it was the first time that BD-CHO was used for visualizing exogenous FA changes in Daphnia magna through fluorescence microscopy, demonstrating its potential application for studies of biological processes associated with FA.