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论文类型：期刊论文

发表时间：2021-06-05

发表刊物：PROCESS BIOCHEMISTRY

卷号：98

页面范围：247-253

ISSN号：1359-5113

关键字：Inulinase; Klebsiella pneumoniae; 2,3-Butanediol; Jerusalem artichoke; High sugar tolerance

摘要：Inulin could be converted to bio-based chemicals by an inulinase producer without external inulinase, and the production of 2,3-butanediol was less than 50 g/L. In this work, a novel inulinase producer of Klebsiella pneumoniae H3 was isolated, and inulinase catalytic properties as well as 2,3-butanediol fermentation were investigated. The enzyme was an intracellular inulinase with an optimal pH of 6 similar to 7 and a temperature of 30 degrees C. The use of inulin by H3 was dependent on the degree of polymerization (DP), and the average DP of inulin in fermentation broth increased from 2.82 to 8.08 in 24-h culture of batch fermentation. Acidic pretreatment was developed to increase inulin utilization by adjusting medium pH to 3.0 prior to sterilization. In batch fermentation with optimized medium and fermentation conditions, the concentration of target product (2,3-butanediol and acetoin) was 80.4 g/L with a productivity of 2.23 g/(L.h), and a yield of 0.426 g/g inulin.