教授 博士生导师 硕士生导师
主要任职: 医学部党委书记兼常务副部长
性别: 男
毕业院校: 复旦大学
学位: 博士
所在单位: 生物医学工程学院
学科: 生物医学工程
电子邮箱: krqin@dlut.edu.cn
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论文类型: 期刊论文
发表时间: 2019-11-01
发表刊物: JOURNAL OF MICROMECHANICS AND MICROENGINEERING
收录刊物: SCIE
卷号: 29
期号: 11
ISSN号: 0960-1317
关键字: intracellular calcium response; dynamic mechanical stimuli; mechanotransduction; cancer cell phenotype; microfluidic device
摘要: To probe intracellular calcium response while single cells squeeze through narrow channels, we built a high-throughput microfluidic device where single cells can be trapped efficiently and stimulated mechanically. With this device, dozens of single cells' dynamic morphologies and intracellular [Ca2+] responses under dynamic mechanical stimuli can be monitored simultaneously. We observed a two-peak [Ca2+] response, which was closely coupled together with the dynamic cellular squeezing process. This type of [Ca2+] response, to our knowledge, was observed for the first time. We also investigated the role of the cytoskeleton in the [Ca2+] response and found that the cytoskeleton was an important regulator of [Ca2+] signaling during the cellular squeezing process. In addition, we investigated the difference between the two-peak [Ca2+] responses of Hela cells and HUVECs and found that one characteristic parameter could distinguish Hela cells from HUVECs.