点击次数：

论文类型：期刊论文

发表时间：2014-07-01

发表刊物：JOURNAL OF FLUORESCENCE

收录刊物：SCIE、EI、Scopus

卷号：24

期号：4

页面范围：1313-1317

ISSN号：1053-0509

关键字：Inorganic mercury; Neuron; Fluorescence probe

摘要：In the present study, we used the previously developed fluorescence probe, EPNP, to generate the first image of the distribution of mercuric ion in primary mouse neuron cultures. At postnatal day 1 (P1), the mice were intraperitoneally (IP) injected with mercuric chloride in doses ranging from 0.05 to 0.6 mu g/g body weight. After 1, 2, 3, and 4 days exposure, primary nervous cell cultures and frozen brain and spinal tissue sections were prepared and dyed using EPNP. On the third day of repeated injections, Hg2+ was visualized in primary cerebral neuron cultures as an increase of Hg2+-induced fluorescence at the doses a parts per thousand yenaEuro parts per thousand 0.1 mu g/g. A similar accumulation of Hg2+ was observed in frozen hippocampus tissue sections. In contrast, no Hg2+ was observed in spinal cord neurons and spinal tissue sections. The detection of a low dose of IP injected mercury in mouse cerebral neurons facilitated the evaluation of the exposure risk to low-dose Hg2+ in immature organisms. Moreover, the highly sensitive EPNP revealed Hg2+ in the cerebral neurons of mice younger than P4, while the presence of Hg2+ was not detected until a parts per thousand yenaEuro parts per thousand P11 in previous reports. Thus, this technology and the results obtained herein are of interest for neurotoxicology.