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论文类型：期刊论文

发表时间：2016-11-09

发表刊物：BMC BIOTECHNOLOGY

收录刊物：SCIE、EI、PubMed、Scopus

卷号：16

期号：1

页面范围：78

ISSN号：1472-6750

关键字：iPSC; Chondrocyte; Osteoarthritis; Differentiation; Transplantation

摘要：Background: The incapacity of articular cartilage (AC) for self-repair after damage ultimately leads to the development of osteoarthritis. Stem cell-based therapy has been proposed for the treatment of osteoarthritis (OA) and induced pluripotent stem cells (iPSCs) are becoming a promising stem cell source.
   Results: Three steps were developed to differentiate human iPSCs into chondrocytes which were transplanted into rat OA models induced by monosodium iodoacetate (MIA). After 6 days embryonic body (EB) formation and 2 weeks differentiation, the gene and protein expression of Col2A1, GAG and Sox9 has significantly increased compare to undifferentiated hiPSCs. After 15 weeks transplantation, no immune responses were observed, micro-CT showed gradual engraftment and the improvement of subchondrol plate integrity, and histological examinations demonstrated articular cartilage matrix production.
   Conclusions: hiPSC could be an efficient and clinically translatable approach for cartilage tissue regeneration in OA cartilages.