宋克东
开通时间:..
最后更新时间:..
点击次数:
论文类型:期刊论文
发表时间:2016-10-01
发表刊物:ANIMAL CELLS AND SYSTEMS
收录刊物:SCIE、Scopus
卷号:20
期号:5
页面范围:276-281
ISSN号:1976-8354
关键字:Bio-derived scaffold; osteoblast; fabrication; bioreactor; repair
摘要:Fabrication and transplantation of tissue-engineered bones in a rotating wall vessel bioreactor (RWVB) was studied in the present study aiming to repair segmental bone defects. Osteoblasts were transfected with green fluorescent protein prior to seeding on bio-derived porous bone scaffolds at a density of 1x10(6)cells/mL and cultured in an RWVB for one week. For comparison, constructs were also cultured in a static condition. Morphology and structure of fabricated bones were examined using an inverted microscope, scanning electron microscope and histology analysis via hematoxylin-eosin and toluidine blue staining. Moreover, an animal model for repairing segmental bone defects of a Zelanian rabbit was used to assess the efficacy and biosafety of fabricated bones. In conclusion, tissue-engineered bones grew favorably in RWVB. In animal study, a preliminary repair of bone defects was noticed only in the experimental group after 4 weeks of implantation. Using RWVB, the fabricated tissue-engineered bone constructs were approved with better bio-capability in repairing the segmental bone defect.