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Indexed by:会议论文

Date of Publication:2016-01-01

Included Journals:CPCI-S

Page Number:296-304

Abstract:Bone marrow mesenchymal stem cells (BMSCs), having the potentials of multi-differentiation, are the promising "seed cells" in tissue engineering and excellent tools for gene therapy. However, BMSCs are in small quantity in bone marrow and difficult to meet clinical need. In this paper, BMSCs mixed with type I collagen were inoculated into hollow fiber membranes (HFMs), where collagen gel with BMSCs formed after an hour at 37 degrees C, 5% CO2 and saturated humidity and then were inoculated into an air-lift loop hollow fiber membrane bioreactor (ALHFMB) for culture in an incubator. During the culture, the cell growth curves and morphology were detected, and metabolic parameters, such as the concentrations of glucose, lactic acid, glutamine, ammonia, O-2, and CO2 as well as pH in medium were also evaluated. Moreover, the expanded cells were identified by phenotypes and multi-differentiation potentials. The results showed that in the ALHFMB, BMSCs metabolized robustly and expanded about 17- and 13-fold at the initial density of 5x10(5) cells.mL(-1) within 7 days in dynamic and static modes, respectively; all the growing cells spread with a spindle appearance and long cell protrusions in gel; the expanded cells still maintained their own phenotypes and had the potentials of multi-differentiation.