Release Time:2019-03-12  Hits:

Indexed by: Journal Article

Date of Publication: 2017-09-22

Journal: INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS

Included Journals: Scopus、EI、SCIE

Volume: 66

Issue: 14

Page Number: 748-752

ISSN: 0091-4037

Key Words: Cell adhesion; chemical cross-linking; copolymer; NIPAAm; temperature-sensitive

Abstract: A novel thermosensitive P (CS-g-TMSPM-g-NIPAAm) copolymer film was synthesized through grafting thermosensitive monomer N-isopropyl acrylamide (NIPAAm) onto the beforehand modified chitosan (CS) by cross-linking agent 3-trimethoxysilypropyl methacrylate (TMSPM). Fourier transform infrared spectroscopy confirmed the successful polymerization of NIPAAm with CS-g-TMSPM, and its lower critical solution temperature was measured at 34 degrees C. Although osteoblasts and adipose-derived stem cells showed excellent growth state on the film in vitro, only osteoblasts demonstrated good adhesion and stretch probably because of the necessary specific cell-film interplay. Hence, for specific cell cultures and nonenzymatic harvesting in vitro, the prepared thermosensitive film could be a promising approach.