Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2011-07-25

Journal: NEURAL REGENERATION RESEARCH

Included Journals: CSCD、SCIE、Scopus

Volume: 6

Issue: 16

Page Number: 1238-1242

ISSN: 1673-5374

Key Words: magnetic resonance imaging; glioma; gene therapy; cytosine deaminase gene; bone marrow mesenchymal stem cells; lentivirus

Abstract: Bone marrow mesenchymal stem cells were isolated from C57BL mice, transfected with the cytosine deaminase (CD) gene using a lentivirus vector and co-cultured with C6 glioma cells to verify anti-tumor effects of bone marrow mesenchymal stem cells carrying CD genes. C57MSC-CD/eGFP cells converted 5-fluorocytosine to 5-fluorouracil and exhibited significant inhibition of proliferation and apoptosis in C6 glioma cells. C57MSC-CD/eGFP cells were then implanted into rat models of brain C6 glioma. Rats were also intraperitoneally injected with 5-fluorocytosine after 7 days. MSC-CD/eGFP cells were irregularly distributed at the margin of the glioma, as well as encased and reduced the volume of the glioma. CD-transfected bone marrow mesenchymal stem cells inhibit the in vivo growth and in vitro proliferation of glioma.