location: Current position: Home >> Scientific Research >> Paper Publications

ADSCs-壳聚糖/明胶水凝胶工程化软骨的三维动态构建

Hits:

Indexed by:期刊论文

Date of Publication:2015-08-15

Journal:功能材料

Included Journals:EI、PKU、ISTIC、CSCD、Scopus

Volume:46

Issue:15

Page Number:15021-15030

ISSN No.:1001-9731

Key Words:转瓶;组织工程软骨;脂肪干细胞;壳聚糖/明胶水凝胶;细胞培养

Abstract:组织工程软骨的体外构建被认为是一种有希望治疗关节软骨缺损的有效途径.为评估载脂肪干细胞(Adipose-derived stem cells,ADSCs)壳聚糖/明胶水凝胶支架,在体外动态构建组织工程软骨相对传统静态培养的优势,本研究用壳聚糖/明胶制备了软骨仿生支架,并检测其物理性质.在制备的水凝胶支架上以1×107 cells/mL 密度接种 ADSCs 后,分别置于转瓶及 T-瓶的软骨诱导基中培养两周,通过试剂染色、代谢检测和电镜观察,考察了细胞的软骨分化能力、活性、生长分布、渗透深度、增殖及胞外基质分泌情况.结果表明,壳聚糖/明胶支架的平均孔径为118.25±19.51μm,孔隙率为82.60±2.34%,吸水率为361.28±0.47%,弹性模量为61.2±0.16 kPa,具有良好生物相容性.ADSCs 生长状态良好,可向软骨细胞分化,适于作为组织工程软骨构建的种子细胞.表征结果显示,转瓶内水凝胶支架中细胞蛋白多糖的表达更显著,细胞生长分布更加均匀,细胞外基质分泌基本填满整个支架.因此,转瓶载壳聚糖/明胶支架所提供的三维动态环境,是体外构建组织工程软骨的良好方法.

Pre One:Three-dimensional dynamic fabrication of engineered cartilage based on chitosan/gelatin hybrid hydrogel scaffold in a spinner flask with a special designed steel frame

Next One:Numberical simulation of fluid flow and three-dimensional expansion of tissue engineering seed cells in large scale inside a novel rotating wall hollow fiber membrane bioreactor