论文名称：Development of a novel europium(III) complex-based luminescence probe for time-resolved luminescence imaging of the nitric oxide production in neuron cells
论文类型：期刊论文
发表刊物：TALANTA
收录刊物：SCIE、EI、PubMed、Scopus
卷号：99
页面范围：951-958
ISSN号：0039-9140
关键字：Europium complex; Luminescence probe; Cell imaging; Nitric oxide; Time-resolved luminescence
摘要：Nitric oxide (NO) is a signaling molecule, and plays an important role in some human neuroses. In this work, a novel europium(III) complex, [4'-(3-methylamino-4-aminophenoxy)-2,2':6',2 ''-terpyridine-6,6 ''-diyl] bis(methylenenitrilo) tetrakis(acetate)-Eu3+ (MATTA-Eu3+), has been designed and synthesized as a luminescent probe for the time-resolved luminescence detection of NO. The complex itself is weakly luminescent, but can specifically react with NO under the aerobic conditions to form its triazole derivative, [4'-(3-methyl-4-benzotriazol-6-yl-oxy)-2,2':6',2 ''-terpyridine-6,6 ''-diyl) bis(methylenenitrilo) tetrakis(acetate)-Eu3+ (MBTTA-Eu3+), accompanied by a remarkable luminescence enhancement with a long luminescence lifetime. The luminescence response of the complex to NO is rapid, highly selective and sensitive, and pH-insensitive in aqueous solutions. These properties enable MATTA-Eu3+ to be used as a turn-on luminescent probe for the selective recognition and sensitive time-resolved luminescence detection of NO in a wider pH range. The probe-loaded PC12 cells were prepared, and used for the time-resolved luminescence imaging detection of the glutamate-induced NO production in the cells. The result demonstrated the practical utility of the probe for imaging the NO production in neuron cells. (C) 2012 Elsevier B.V. All rights reserved.
发表时间：2012-09-15