论文名称：Preparation of visible-light-excited luminescence enhancement solutions for time-resolved luminescence detection of europium biolabel
论文类型：期刊论文
发表刊物：ANALYST
收录刊物：SCIE、PubMed、Scopus
卷号：137
期号：19
页面范围：4502-4508
ISSN号：0003-2654
摘要：Dissociation enhanced lanthanide fluoroimmunoassay (DELFIA) technique based on EDTA-Eu3+ derivative biolabels is the most widely used time-resolved luminescence bioassay technique for clinical diagnosis, but its major drawback is that the conventional luminescence enhancement solution of EDTA-Eu3+ requires UV excitation (<360 nm). In this work, three new visible-light-excited luminescence enhancement solutions are developed and their luminescence response behaviors to EDTA-Eu3+ are systematically investigated. The new solutions were prepared by co-dissolving a newly synthesized tetradentate beta-diketone, 1,2-bis[8'-(1 '',1 '',1 '',2 '',2 '',3 '',3 ''-heptafluoro-4 '',6 ''-hexanedion-6 '' yl)-naphth-2'-yl]-benzene (BHHNB), and one of three derivatives of triazine, 2-(N,N-diethylanilin-4-yl)-4,6-bis(3,5-dimethylpyrazol-1-yl)-1,3,5-triazine (DPBT), 2-(N,N-diethylanilin-4-yl)-4,6-bis(3-methylpyrazol-1-yl)-1,3,5-triazine (MPBT) or 2-(N,N-diethylanilin-4-yl)-4,6-bis(pyrazol-1-yl)-1,3,5-triazine (BPT), in a weakly acidic aqueous buffer at pH 3.2 containing 0.1% Triton X-100. These solutions showed sensitive and rapid luminescence responses to non-luminescent EDTA-Eu3+ by the formation of the ternary Eu3+ complexes, BHHNB-Eu3+-DPBT, BHHNB-Eu3+-MPBT and BHHNB-Eu3+-BPT. These complexes have long luminescence lifetimes (>500 mu s) and a wide excitation wavelength range from UV to visible light with the excitation peaks at 390, 400 and 420 nm, respectively, which enabled the solutions to be used as visible-light-excited luminescence enhancement solutions for the highly sensitive time-resolved luminescence detection of EDTA-Eu3+.
发表时间：2012-10-07