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论文类型：期刊论文

发表时间：2015-07-01

发表刊物：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

收录刊物：SCIE、EI、PubMed、Scopus

卷号：62

期号：4

页面范围：530-536

ISSN号：0885-4513

关键字：Box-Behnken design; cell encapsulation; islet survival rate; islet transportation; response surface methodology

摘要：Restoration of insulin secretion by transplantation of isolated islets is a treatment for type diabetes mellitus. One of the major issues with clinical treatment of islet transplantation is how to maintain islet viability during transportation from the donor to the patient. We developed a method that uses alginate encapsulation to protect islets from mechanical damage during shipment. We tested several variables for their impact on islet viability during transportation and used the significant variable to build a response surface methodology (RSM) model by the Box-Behnken design method. This type of model is a mathematical and statistical technique that we used to optimize the conditions for islet viability during shipment. In this study, the factors that significantly affected islet survival rate were incubation time, serum concentration, and preservation time. Then, an empirical model was built to optimize conditions of the islets for shipping according to the responses of the effect factors with RSM. This model can be used to predict the islet survival rate and can serve as a guide for optimizing the transportation method of islets and increasing the success rate of the transplant procedure.