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论文类型：会议论文

发表时间：2021-01-10

卷号：11190

关键字：continuous infrared laser; cell membrane capacitance; inactivation of Sodium channel; neural stimulation; neural inhibition

摘要：Infrared laser had been proved their effectiveness on injury repairing, synchronize embryos and neural regulation etc. Pulsed laser with wavelengths >similar to 1.5 mu m was used in controlling nerves in vitro, such as stimulation or block action potentials. Theoretical analysis demonstrated that whether enhancement or inhibition could be ascribed to the thermal effect induced by the pulsed laser. A membrane capacitance mediation mechanism had been proved to act as a chromophore in the mediation this thermal effect with the cell function. Here, we explored a continuous infrared laser irradiation situation, which was a case in clinic. A continuous near infrared laser with a wavelength centered at 980nm was employed to irradiate a neuron cell in vitro. And a patch clamp technique was employed to record a whole cell current. The whole cell current was formed by a compensated capacitance current, a rapid voltage-dependent inward sodium current, and a slowly voltage-dependent outward-rectifier potassium current. After a 36s irradiation, the potassium current was changed not that much, but the sodium current was decreased in its peak current. We analyzed the compensated capacitance current and found that the infrared irradiation decreased the membrane capacitance also. Analysis demonstrated that this can also be related to temperature raising gradient. Our results support that a near infrared continuous laser change neurons activities by reducing its membrane capacitance.