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论文类型：期刊论文

发表时间：2009-01-01

发表刊物：CELL BIOCHEMISTRY AND BIOPHYSICS

收录刊物：SCIE、PubMed

卷号：53

期号：1

页面范围：33-42

ISSN号：1085-9195

关键字：Voltage-dependent ion channels; Activation; Inactivation; Action potential; Laser; Temperature

摘要：Optical control of neuronal activity has a number of advantages over electrical methods and can be conveniently applied to intact individual neurons in vivo. In this study, we demonstrated an experimental approach in which a focused continuous near-infrared (CNI) laser beam was used to activate single rat hippocampal neurons by transiently elevating the local temperature. Reversible changes in the amplitude and kinetics of neuronal voltage-gated Na and K channel currents were recorded following irradiation with a single-mode 980 nm CNI-laser. Using single-channel recordings under controlled temperatures as a means of calibration, it was estimated that temperature at the neuron rose by 14A degrees C in 500 ms. Computer simulation confirmed that small temperature changes of about 5A degrees C were sufficient to produce significant changes in neuronal excitability. The method should be broadly applicable to studies of neuronal activity under physiological conditions, in particular studies of temperature-sensing neurons expressing thermoTRP channels.