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Indexed by:期刊论文

Date of Publication:2018-01-17

Journal:ACS APPLIED MATERIALS & INTERFACES

Included Journals:SCIE、EI、PubMed

Volume:10

Issue:2

Page Number:1499-1507

ISSN No.:1944-8244

Key Words:Pim-1 kinase; biomarkei-targeting; cancer cell imaging mitochondria localization; in vivo imaging

Abstract:Based on the fact that enzyme-targeting probes are highly sensitive and selective, a novel red-emitting probe (NB-BF) for Pim-1 kinase including three parts, fluorophore (NB), linker, and inhibitor (BF), has been designed for cancer optical imaging. In its free state, NB BF is folded and the fluorescence quenched by PET between fluorophore and inhibitor both in PBS buffer and in normal cells. Significantly, it emitted strong red fluorescence in Pim-1 overexpressed cancer cells. The specificity of NB-BF for Pim-1 kinase was directly demonstrated by gene silencing analysis. Furthermore, it is the first time to know where Pim-1 kinase mainly distributes at mitochondria with Pearson's correlation factor (R-r) of 0.965 and to provide a fluorescent tool to verify the function of the Pim-1 kinase. More importantly, NB-BF was applied in tissue imaging and preferentially labeled tumors in vivo.