Hits:
Indexed by:期刊论文
Date of Publication:2018-01-01
Journal:ACS sensors
Included Journals:PubMed、SCIE、EI
Volume:3
Issue:3
Page Number:735-741
ISSN No.:2379-3694
Key Words:superoxide anion; mitochondria-accessing; ratiometric bioimaging; fluorescent probe; fast response; inflammatory Daphnia magna
Abstract:Superoxide anion (O2-), as the precursor of other reactive oxygen species (ROS), is significantly important in the maintenance of redox homeostasis and various cellular signaling pathways. Here we present a ratiometric mitochondria-accessing fluorescent probe (NA-T) based on nucleophilic substitution mechanism for real-time measuring O2-. By regulating the intramolecular charge of 1,8-naphthalimide, a ratiometric response model was obtained, which evinced 18-fold enhancement of fluorescence ratio ( I540nm/ I475nm) in the presence of O2- over other ROS with rapid response (132 s), high sensitivity (DL = 0.370 muM) and selectivity. Confocal fluorescence images demonstrated that the probe could well permeate through plasma membrane for visualizing endogenous O2- changes in mitochondria of living cells and in inflammatory Daphnia magna, indicating NA-T a potential tool for the diagnosis and research of corresponding diseases.
Pre One:Conformationally Induced Off-On Cell Membrane Chemosensor Targeting Receptor Protein-Tyrosine Kinases for in Vivo and in Vitro Fluorescence Imaging of Cancers.
Next One:Visualization of methylglyoxal in living cells and diabetic mice model with a 1,8-naphthalimidebased two-photon fluorescent probe
