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Indexed by:期刊论文

Date of Publication:2017-10-01

Journal:CHINESE CHEMICAL LETTERS

Included Journals:SCIE、Scopus

Volume:28

Issue:10,SI

Page Number:1997-2000

ISSN No.:1001-8417

Key Words:Hypoxia; Fluorescence probes; Nitroreductase; Near-infrared; Aminocyanine dyes

Abstract:Imaging hypoxia using fluorescence probes for nitroreductase (NTR) have attracted much attention in last decade. At least three different linkers have been commonly used to connect the recognition unit and reporting unit in reported probes for NTR. Meanwhile, the linker is known to be a key factor for achieving best sensing performance. In this work, three near-infrared fluorescence probes CyNP-1, CyNP-2 and CyNP-3 were designed and synthesized from an aminocyanine dye CyNP. The three probes have the same recognition unit and same fluorescence reporting unit, but different linkers. CyNP-1 was found to have the best sensing performance for NTR with 40-fold of fluorescence enhancement. It is well investigated how the difference of the linkers brings out the different sensing performance by HPLC, MS and docking calculations. In the end, CyNP-1 was found to have good selectivity for NTR and used to imaging hypoxia in Hela cells. (C) 2017 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.