Release Time:2019-03-12  Hits:

Indexed by: Journal Article

Date of Publication: 2018-04-01

Journal: SCIENCE CHINA-CHEMISTRY

Included Journals: SCIE

Volume: 61

Issue: 4

Page Number: 468-475

ISSN: 1674-7291

Key Words: nucleic acids; ctDNA; fluorescent probe; thiazole orange

Abstract: The sensitive and selective detection of nucleic acids is important for basic research and many applied fields. Herein, a novel green DEAB-TO-1 was designed and synthesized. DEAB-TO-1 shows significant fluorescence enhancement (800-fold) when bound to ctDNA and a distinct selectivity for dsDNA over RNA (5.9 times). Moreover, the limit of detection is 0.57 ng/mL. It is live-cell-permeant DNA intecalator, indicating a promising candidate for nucleus-specific imaging and a good counter-stain compatibility with other dyes.