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Indexed by:期刊论文

Date of Publication:2018-04-01

Journal:SCIENCE CHINA-CHEMISTRY

Included Journals:SCIE

Volume:61

Issue:4

Page Number:468-475

ISSN No.:1674-7291

Key Words:nucleic acids; ctDNA; fluorescent probe; thiazole orange

Abstract:The sensitive and selective detection of nucleic acids is important for basic research and many applied fields. Herein, a novel green DEAB-TO-1 was designed and synthesized. DEAB-TO-1 shows significant fluorescence enhancement (800-fold) when bound to ctDNA and a distinct selectivity for dsDNA over RNA (5.9 times). Moreover, the limit of detection is 0.57 ng/mL. It is live-cell-permeant DNA intecalator, indicating a promising candidate for nucleus-specific imaging and a good counter-stain compatibility with other dyes.