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Indexed by:期刊论文

Date of Publication:2016-07-15

Journal:JOURNAL OF CHROMATOGRAPHY A

Included Journals:SCIE、EI、PubMed

Volume:1455

Page Number:147-155

ISSN No.:0021-9673

Key Words:Glycoprotein/glycopeptide; Lectin affinity chromatography; Mass spectrometry; Oxidized dextran

Abstract:Lectin affinity chromatography (LAC) is an important enrichment technique in glycoproteomics analysis. In order to improve the effectiveness of enrichment, it is necessary to develop LAC materials with high specificity and efficiency. Herein, using oxidized dextran as the spacer, a silica-based concanavalin A material (SiO2-ODex Con A) was synthesized to enrich glycoproteins/glycopeptides. For comparison, the SiO2-Ald Con A material was synthesized using conventional (3-glycidoxypropyl) triethoxysilane (GPMS) as the initial spacer arm. The analytical merits of both Con A materials, such as non-specific adsorption, binding capacity and trapping efficiency, have been evaluated using ovalbumin. Under high performance liquid affinity chromatography (HPLAC) mode, the SiO2-ODex Con A material was highly effective in the enrichment of glycoproteins/glycopeptides attached to high-mannose-type and bi-antennary complex type glycans. The promising potential of the SiO2-ODex Con A material was demonstrated by selective fractionation of glycoproteins from complex biological samples for glycosylation analysis. (C) 2016 Elsevier B.V. All rights reserved.