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Indexed by:期刊论文

Date of Publication:2016-04-01

Journal:JOURNAL OF SEPARATION SCIENCE

Included Journals:SCIE、EI、PubMed

Volume:39

Issue:8

Page Number:1461-1470

ISSN No.:1615-9306

Key Words:Basic compounds; High-performance liquid chromatography; Michael addition; Stationary phases; Thiol-yne click chemistry

Abstract:A novel silica-based stationary phase with branched octadecyl groups was prepared by the sequential employment of the Michael addition reaction and photoinduced thiol-yne click chemistry with 3-aminopropyl-functionalized silicamicrospheres as the initialmaterial. The resulting stationary phase denoted as SiO2-N(C18)(4) was characterized by elemental analysis, FTIR spectroscopy and Raman spectroscopy, demonstrating the existence of branched octadecyl groups in silicamicrospheres. The separations of benzene homologous compounds, acid compounds and amine analogues were conducted, demonstratingmixed-mode separation mechanism on SiO2-N(C18)(4). Baseline separation of basic drugs mixture was acquired with the mobile phase of acetonitrile/H2O (5%, v/v). SiO2-N(C18)(4) was further applied to separate Corydalis yanhusuo Wang water extracts, and more baseline separation peaks were obtained for SiO2-N(C18)(4) than those on Atlantis dC18 column. It can be expected that this new silica-based stationary phase will exhibit great potential in the analysis of basic compounds.