Release Time:2019-03-13  Hits:

Indexed by: Journal Article

Date of Publication: 2016-01-22

Journal: SCIENTIFIC REPORTS

Included Journals: PubMed、SCIE

Volume: 6

Page Number: 19562

ISSN: 2045-2322

Abstract: Lysosome-specific fluorescent probes are exclusive to elucidate the functions of lysosomal thiols. Moreover, two-photon microscopy offers advantages of less phototoxicity, better three dimensional spatial localization, deeper penetration depth and lower self-absorption. However, such fluorescent probes for thiols are still rare. In this work, an efficient two-photon fluorophore 1,8-naphthalimide-based probe conjugating a 2,4-dinitrobenzenesulfonyl chloride and morpholine was designed and synthesized, which exhibited high selectivity and sensitivity towards lysosomal thiols by turn-on fluorescence method quantitatively and was successfully applied to the imaging of thiols in live cells and tissues by two-photon microscopy.