Release Time:2022-04-11  Hits:

Indexed by: Journal Article

Date of Publication: 2021-02-02

Journal: ACS SENSORS

Volume: 5

Issue: 10

Page Number: 3158-3164

ISSN: 2379-3694

Key Words: singlet oxygen; chemiluminescence imaging; near-infrared; photodynamic therapy; phototoxicity; mice model

Abstract: Singlet oxygen (O-1(2)) plays a vital role in metabolism. However, because of its extremely high reactivity and short-lived state, the in vivo detection of O-1(2) is challenging. To address this issue, for the first time, we herein constructed a near-infrared (NIR) chemiluminescent probe (CL-SO) by caging the precursor of phenoxy-dioxetane scaffolds and a dicyanomethyl-chromone acceptor for selective O-1(2) detection. This probe can detect (1)O(2 )in vitro with a tremendous turn-on chemiluminescence signal in the NIR region (700 nm) and image intracellular O-1(2) produced by the photosensitizer during the simulated action of photodynamic therapy (PDT). Notably, (1)O(2 )level changes in the abdominal cavity and tumor of the various mice model under different stimulations and PDT action were effectively monitored by CL-SO, providing a novel chemiluminescence imaging platform to explore O-1(2) generation in PDT-associated applications.