Hits:

Indexed by:期刊论文

Date of Publication:2004-07-01

Journal:CELL BIOLOGY INTERNATIONAL

Included Journals:Scopus、SCIE、PubMed

Volume:28

Issue:4

Page Number:287-291

ISSN No.:1065-6995

Key Words:triethylene tetraamine; telomerase; cell cycle; proliferation

Abstract:Telomerase, which is required to maintain telomeres, has attracted considerable attention as a target for anticancer therapy. In this study, we investigated the inhibition of HeLa cell telomerase activity and cell cycle progression by triethylene tetraamine (TETA), using a modified telomeric repeat amplification protocol (TRAP) assay, and flow cytometry. TETA inhibited telomerase activity in HeLa cell extracts, with an IC50 of about 7.8 muM. Coupled with this inhibition, TETA also increased the proportion of cells in the G(1) phase of the cell cycle in a dose-dependent manner. These findings demonstrate that TETA is a potent inhibitor of telomerase in micromolar concentrations, and inhibits the proliferation of HeLa cells by arresting them in G(1). (C) 2004 Elsevier Ltd. All rights reserved.