王竞

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教授

博士生导师

硕士生导师

性别:男

毕业院校:哈尔滨工业大学

学位:博士

所在单位:环境学院

办公地点:环境楼B611

联系方式:13940939399

电子邮箱:jwang@dlut.edu.cn

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Optimization of butanol production from corn straw hydrolysate by Clostridium acetobutylicum using response surface method

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论文类型:期刊论文

发表时间:2011-05-01

发表刊物:CHINESE SCIENCE BULLETIN

收录刊物:SCIE

卷号:56

期号:14

页面范围:1422-1428

ISSN号:1001-6538

关键字:enzymatic hydrolysis; butanol fermentation; Plackett-Burman design; response surface method

摘要:Butanol is a new kind of very potential biofuels. Enzymatic hydrolysis of corn stalk was utilized in this study to produce butanol by Clostridium acetobutylicum CICC 8008. Plackett-Burman (P-B) design and Central Composite Design (CCD) were adopted to screen crucial factors during fermentation as well as the optimization of experimental conditions. The result demonstrated that among the seven factors, namely, Yeast extract, (NH4)(2)SO4, KH2PO4, MgSO4, FeSO4, CuSO4 and CaCO3, only CaCO3 was selected as the most critical factor. The optimization experiment results for CaCO3 usage, temperature and reaction time by CCD were determined to be 5.04 g/L, 35A degrees C and 70 h, respectively. A corresponding mathematical model was established to predict the fermentation experiment and maximum butanol yield of 6.57 g/L was acquired. The result of verification experiment under the optimum conditions showed that 6.20 g/L was the maximum butanol yield. This demonstrated that statistical method was a powerful tool for the optimization of butanol production from enzymatic hydrolysis of corn stalk.