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传统发酵豆酱中产蛋白酶菌株的筛选

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Indexed by:期刊论文

Date of Publication:2015-07-15

Journal:中国酿造

Included Journals:PKU、ISTIC

Volume:34

Issue:7

Page Number:32-35

ISSN No.:0254-5071

Key Words:豆酱中的真菌;蛋白酶活性;米曲霉

Abstract:该研究从东三省各地采集家庭自制豆酱254份,通过分离培养,获得纯培养真菌菌株389株.采用脱脂牛奶平板初筛获得具有蛋白酶活性的菌株31株,其中曲霉属真菌12株、青霉5株、毛霉4株、镰孢菌3株、帚霉1株、芽枝孢3株、犁头霉1株、红曲2株.通过发酵复筛提取其粗酶液,用福林酚法测定粗酶液的蛋白酶活性,筛选出了两株具有较高蛋白酶活性的米曲霉.

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