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传统发酵豆酱中产蛋白酶菌株的筛选

Release Time:2019-03-10  Hits:

Indexed by: Journal Article

Date of Publication: 2015-07-15

Journal: 中国酿造

Included Journals: ISTIC、PKU

Volume: 34

Issue: 7

Page Number: 32-35

ISSN: 0254-5071

Key Words: 豆酱中的真菌;蛋白酶活性;米曲霉

Abstract: 该研究从东三省各地采集家庭自制豆酱254份,通过分离培养,获得纯培养真菌菌株389株.采用脱脂牛奶平板初筛获得具有蛋白酶活性的菌株31株,其中曲霉属真菌12株、青霉5株、毛霉4株、镰孢菌3株、帚霉1株、芽枝孢3株、犁头霉1株、红曲2株.通过发酵复筛提取其粗酶液,用福林酚法测定粗酶液的蛋白酶活性,筛选出了两株具有较高蛋白酶活性的米曲霉.

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