A wash-free SNAP-tag fluorogenic probe based on the additive effects of quencher release and environmental sensitivity
Release Time:2021-03-15 Hits:
Indexed by: Journal Article
Date of Publication: 2021-01-29
Journal: CHEMICAL COMMUNICATIONS
Volume: 53
Issue: 48
Page Number: 6448-6451
ISSN: 1359-7345
Abstract: A 1,8-naphthalimide-derived fluorogenic probe was reported to label SNAP-tag fusion proteins in living cells. The probe can rapidly label a SNAP-tag and exhibit a fluorescence increase of 36-fold due to the additive effects of environment sensitivity of fluorophores and inhibition of photo-induced electron transfer from O-6-benzylguanine to the fluorophore. The labeling of intracellular proteins has been successfully achieved without a wash-out procedure.