A fluorescent probe based on N-butylbenzene-1,2-diamine for Cu(II) and its imaging in living cells
Release Time:2019-03-09 Hits:
Indexed by: Journal Article
Date of Publication: 2014-12-01
Journal: JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY
Included Journals: SCIE
Volume: 80
Issue: 3-4
Page Number: 383-390
ISSN: 0923-0750
Key Words: 1,8-Naphthalimide; o-N-Butylbenzene-1,2-diamine; Fluorescence; Probe; Copper ion; Live cells imaging
Abstract: A fluorescent probe LZ-N with naphthalimide as fluorophore and N-butylbenzene-1,2-diamine as a new recognition moiety for copper ion was designed and synthesized. The probe LZ-N exhibits high selectivity for Cu2+ ion in aqueous media (CH3CN:H2O = 1:1) over all the other metal ions in our study, more than 20-fold fluorescence enhancement by coordinating with Cu2+, and the maximum emission intensity independence in the range of pH 2.06-9.25. The results of H-1-NMR titration, time-resolved fluorescence decay measurement, and computational optimization illuminate the mechanisms of Cu2+ and probe LZ-N. Confocal fluorescence images and cell viability values test show the high fluorescence enhancement of probe LZ-N for exogenous Cu2+ in living cells.