Hits:

Indexed by:期刊论文

Date of Publication:2014-07-15

Journal:BIOSENSORS & BIOELECTRONICS

Included Journals:SCIE、EI、PubMed、Scopus

Volume:57

Page Number:30-35

ISSN No.:0956-5663

Key Words:Ratiometric fluorescent probe; Human carboxylesterase 1; Large emission shift; Cell imaging

Abstract:A new ratiometric fluorescent probe derived from 2-(2-hydroxy-3-methoxyphenyl) benzothiazole (HMBT) has been developed for selective monitoring of human carboxylesterase 1 (hCE1). The probe is designed by introducing benzoyl moiety to HMBT. The prepared latent spectroscopic probe 1 displays satisfying stability under physiological pH conditions with very low background signal. Both the reaction phynotyping and chemical inhibition assays demonstrated that hCE1 mediated the specific cleavage of the carboxylic ester bond of probe 1 in human biological samples. The release of HMBT leads to a remarkable red-shifted emission in fluorescence spectrum (120 nm large emission shift). Furthermore, human cell-based assays show that probe 1 is cell membrane permeable, and it can be used for bioassay and cellular imaging of hCE1 activity in HepG2 cells. These findings lead to the development of a simple and sensitive fluorescent method for measurement of hCE1 activity in vitro or in living cells, in the presence of additional enzymes or endogenous compounds. (C) 2014 Elsevier B.V. All rights reserved.