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个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：生物工程学院副院长
其他任职：辽宁省生物工程教指委秘书长
性别：女
毕业院校：大连理工大学
学位：博士
所在单位：生物工程学院
学科：生物化工
办公地点：生物楼321
联系方式：0411-84706802
电子邮箱：ywj@dlut.edu.cn
Characterization of inulinase promoter from Kluyveromyces marxianus for intensive protein expression in industrial biotechnology
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论文类型：期刊论文
发表时间：2017-09-01
发表刊物：FEMS YEAST RESEARCH
收录刊物：Scopus、SCIE、PubMed
卷号：17
期号：6
ISSN号：1567-1356
关键字：inulinase; KmINU1 promoter; glucose repression; carbon source; Kluyveromyces marxianus; Mig1
摘要：Inulinase from Kluyveromyces marxianus (KmINU1) has wide application in industrial biotechnology, and it is supposed that its expression is regulated at the transcriptional level via the promoter. Therefore, in this study, the KmINU1 promoter, fused to the reporter EGFP gene, was analyzed to determine its fundamental regulatory mechanisms in Saccharomyces cerevisiae S288C (ATCC 204508). Induction by inulin and repression by high glucose concentrations of KmINU1 promoters are reported, and the promoter with a length of 353 bp was shown to have the highest strength with the weakest responses to high glucose concentration. Responses of the KmINU1 promoter to carbon source were shown to be related to the Mig1 binding site extending from -496 to -485 bp. Hence, we propose clear regulation mechanisms of the inulinase promoter, which may provide a powerful theoretical reference for its application in protein production.
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