点击次数：

发表时间：2009-01-01

发表刊物：分析化学

所属单位：生物工程学院

卷号：37

期号：6

页面范围：850-854

ISSN号：0253-3820

摘要：A high performance liquid chromatographic method was developed for the simultaneous determination of ethanol, sugar and organic acids in Kluveromyces marxinus broth by UV and RI (Refractive index) detector. Aminex HPX-87H organic acids analysis column was used for separation. The mobile phase was composed of sulfuric acid and water, and the concentration of sulfuric acid was 0.01 mol/L (pH 1.4). The flow rate was 0.5 mL/min. The broth (containing glucose, fructose, ethanol, acetic acid, glycerol, and lactic acid) was separated by the dual-channel detector within 25 min. The method showed high average recoveries for the samples ranged from 95.8% to 109.6% and good precision (RSD) ranged from 0.3% to 4.0%. The results show that ethanol, sugar and organic acids can be determined rapidly and effectively, and is capable of being used for real-time analysis of metabolic components in fermentation broth.

备注：新增回溯数据