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论文类型：期刊论文

发表时间：2013-12-01

发表刊物：DYES AND PIGMENTS

收录刊物：SCIE、EI、Scopus

卷号：99

期号：3

页面范围：620-626

ISSN号：0143-7208

关键字：Naphthalimide-rhodamine; FRET; Ratiometric sensing; pH; Fluorescent sensor; Lysosome localization

摘要：In this work, we presented a naphthalimide-rhodamine based fluorescence resonance energy transfer system (FRET) NR1 as a ratiometric and intracellular pH probe, in which 1,2,3-triazole was identified as an ideal bridge and biocompatibility. It could selectively monitor pH variations in methanol/HEPES solution at room temperature. When the pH changed from 6.20 to 2.00, both the fluorescence intensities at 580 nm and the intensity ratios, R (I-580 nm/I-538 nm) increased, which allowed the detection of pH changes by both normal fluorescence and ratiometric fluorescence methods. The observation is consistent with the increased FRET from the 1,8-naphthalimide (donor) to the ring-opened, colored form of rhodamine (acceptor). Moreover, as NR1 is lysosomal with low cytotoxicity, it will be helpful for investigating the pivotal role of H+ in a biological context, especially in lysosomes through direct intracellular imaging. (C) 2013 Elsevier Ltd. All rights reserved.