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个人信息Personal Information
教授
博士生导师
硕士生导师
性别：女
毕业院校：大连理工大学
学位：博士
所在单位：生物工程学院
学科：生物化工. 生物工程与技术. 药物工程
办公地点：生物楼512
联系方式：wangjingyun67@dlut.edu.cn
电子邮箱：wangjingyun67@dlut.edu.cn
Fluorene-derived two-photon fluorescent probes for specific and simultaneous bioimaging of endoplasmic reticulum and lysosomes: group-effect and localization
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论文类型：期刊论文
发表时间：2013-10-28
发表刊物：JOURNAL OF MATERIALS CHEMISTRY B
收录刊物：SCIE、EI、Scopus
卷号：1
期号：40
页面范围：5450-5455
ISSN号：2050-750X
摘要：Two novel fluorene-derived two-photon fluorescent probes (TPFL) targeting the endoplasmic reticulum (TPFL-ER) and lysosomes (TPFL-Lyso) were synthesized by introducing a chlorine group and a morpholine group, respectively. They were shown to be suitable for specific and simultaneous imaging of the endoplasmic reticulum and lysosomes without affecting protein movements owing to their similar molecular structures. TPFL-ER and TPFL-Lyso were successfully used to visualize the changes of the endoplasmic reticulum and lysosomes during cancer cell apoptosis and they demonstrated high specificity and sensitivity, excellent photostability, and low phototoxicity.