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Indexed by:期刊论文

Date of Publication:2008-04-01

Journal:BIORESOURCE TECHNOLOGY

Included Journals:PubMed、SCIE

Volume:99

Issue:6

Page Number:1722-1728

ISSN No.:0960-8524

Key Words:urea nitrogen removal; Anammox; Bacillus strains

Abstract:In this study, the feasibility of biological urea nitrogen removal in anaerobic Anammox co-culture was investigated. After 100 days of operation, complete urea nitrogen removal of 0.35 g (NH2)(2)CO-N L-1 d(-1) was achieved. The pure Anammox bacteria were obtained by percoll density-gradient centrifugation and found to be of incapable to hydrolyze urea. The ureolytic bacteria were isolated from the Anammox co-culture by the spread plate and streak. Comparative analysis of partial 16S rDNA sequence presented it belongs to Bacillus sp., and so named as Bacillus sp. LST-1. Fluorescence in situ hybridization was applied to identify the ratio of Bacillus sp. and Anammox in the reactor and the value was approximately 1:4. Urea nitrogen removal was realized in this autotrophic, anoxic reactor via the combined process of urea hydrolysis by Bacillus sp. LST-1 and ammonium oxidizing by Anammox. The investigation of this combined process might have an actual significance in engineering application for its low operational cost. (C) 2007 Elsevier Ltd. All rights reserved.