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黄芪多糖对仿刺参体腔细胞中溶菌酶基因表达量的影响

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Indexed by:期刊论文

Date of Publication:2009-10-25

Journal:水产科学

Included Journals:PKU

Volume:28

Issue:10

Page Number:572-574

ISSN No.:1003-1111

Key Words:黄芪多糖;仿刺参;溶菌酶;基因表达

Abstract:为研究黄芪多糖诱导仿刺参体腔细胞中溶菌酶基因表达的影响,从体质量(10±1.22) g的仿刺参体腔细胞中克隆出长度为395 bp的β-actin基因部分片段作为内参,利用半定量PCR法考察体内注射不同剂量黄芪多糖(APS)后仿刺参体腔细胞中i 型溶菌酶mRNA表达量变化.研究发现,5、10 mg/kg剂量的APS可提高体细胞中溶菌酶mRNA表达量,其中5 mg/kg剂量组达显著水平,剂量为20 mg/kg时基本无效.

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