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论文类型：期刊论文

发表时间：2016-09-01

发表刊物：ANNALS OF MICROBIOLOGY

收录刊物：SCIE、Scopus

卷号：66

期号：3

页面范围：963-971

ISSN号：1590-4261

关键字：Pediococcus pentosaceus; Gastrointestinal tolerance; Antimicrobial activity; Immunomodulatory activity; Chemokines; Probiotics

摘要：This study aimed to investigate the probiotic potential of Pediococcus pentosaceus L1 isolated from paocai, a Chinese fermented vegetable. In vitro analysis revealed that P. pentosaceus L1 had the capability to tolerate simulated gastrointestinal juices. Adhesion of P. pentosaceus L1 to HT-29 intestinal epithelial cells (IEC) was also observed. L1 was sensitive to ampicillin, gentamycin, kanamycin, strepomycin, clindamycin, tetracycline and chloramphenicol. L1 showed effective inhibition against Escherichia coli, Salmonella typhimurium, and Shigella flexneri. P. pentosaceus L1 also exhibited the abilities of auto-aggregation and co-aggregation with Shigella flexneri. Pre-treatment of HT-29 IEC with P. pentosaceus L1 prior to tumor necrosis factor-alpha (TNF alpha) challenge down-regulated the expression of pro-inflammatory genes, such as IL8, CCL20, CXCL10, and CXCL1. The level of IL-8 released in culture supernatant of TNF alpha-challenged HT-29 IEC was reduced by strain L1, confirming the observed decrease in TNF alpha-induced IL-8 mRNA expression. These results indicate the probiotic potential of P. pentosaceus L1, and that this strain could be used to produce functional foods.