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个人信息Personal Information
教授
博士生导师
硕士生导师
性别：男
毕业院校：加拿大萨斯卡彻温大学
学位：博士
所在单位：生物工程学院
电子邮箱：xyping@dlut.edu.cn
Monitoring Nitric Oxide in Subcellular Compartments by Hybrid Probe Based on Rhodamine Spirolactam and SNAP-tag
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论文类型：期刊论文
发表时间：2016-07-01
发表刊物：ACS CHEMICAL BIOLOGY
收录刊物：SCIE、PubMed、Scopus
卷号：11
期号：7
页面范围：2033-2040
ISSN号：1554-8929
摘要：By connection of O-6-benzylguanine (BG) to an "o-phenylenediamine-locked" rhodamine spirolactam responsive to nitric oxide (NO), a novel substrate (TMR-NO-BG) of genetically encoded SNAP-tag has been constructed. In living cells, labeling SNAP-tag fused proteins with TMR-NO-BG will in situ generate corresponding probe protein conjugates (TMR-NO-SNAP) that not only inherit high NO sensitivity from the small-molecule parent but also guarantee the site-specificity to the designated subcellular compartments such as the mitochondrial inner membrane, nucleus, and cytoplasm. In two representative cellular processes, TMR-NO-BG demonstrates its applicability to monitor endogenous subcellular NO in the activated RAW264.7 cells stimulated by lipopolysaccharide and in the apoptotic COS-7 cells induced by etoposide.
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