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论文类型：期刊论文

发表时间：2008-07-27

发表刊物：VETERINARY MICROBIOLOGY

收录刊物：SCIE、PubMed、Scopus

卷号：130

期号：1-2

页面范围：126-133

ISSN号：0378-1135

关键字：egg yolk immunoglobulin; bovine mastitis; Escherichia coli; growth inhibition; phagocytosis

摘要：The objective of this study was to estimate the in vitro activity of egg yolk immunoglobulin (IgY) against mastitis-causing Escherichia coli. Specific IgY was produced by hens immunized with formaldehyde killed E. coli O111 in long-standing immunization response (titer >= 6400 for 100 days) and was isolated from yolks with a purity of 86% by water dilution, salt precipitations and ultrafiltration. Enzyme-linked inummosorbent assay (ELISA) indicated the produced IgY specifically targeted E. coli O 111 and five other E. coli strains which were isolated from mastitic cows. The growth inhibition activity of the specific IgY to bacteria was dose-dependent with an effective concentration of 20 mg purified IgY per milliliter. The phagocytic activity of E. coli either by milk macrophages (M Phi) or by polymorphonuclear neutrophil leukocytes (PMN) in the presence of specific IgY was significantly higher than that with nonspecific IgY or without IgY (p < 0.05), suggesting that it enhanced phagocytic activity. The current work suggests that this specific IgY has potential as a therapeutic treatment for mastitis in dairy cows. (c) 2007 Elsevier B.V. All rights reserved.