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Indexed by:期刊论文

Date of Publication:2014-08-31

Journal:Journal of Donghua University (English Edition)

Included Journals:EI、Scopus

Volume:31

Issue:4

Page Number:391-395

ISSN No.:16725220

Abstract:Exogenous DNA expressing green fluorescent protein (GFP) and labeled with fluorescein isothiocyanate (FITC) was used to transform the Chinese oak silkmoth Antheraea pernyi(A. pernyi) via sperm-mediated gene transfer (SMGT). Sperms entry into the female reproductive system and eggs were observed using fluorescence microscopy. The ability of A. pernyi sperms to uptake exogenous DNA was confirmed, and transfer of the exogenous DNA was shown by GFP expression in the transgenic eggs. Our result suggested that SMGT could also be used to directly generate transgenic A. pernyi expressing functional genes of interest. Copyright ? 2014 Editorial Department of Journal of Donghua University.