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Date of Publication:2011-01-01

Journal:基因组学与应用生物学

Volume:30

Issue:1

Page Number:16-20

ISSN No.:1008-3464

Abstract:The extreme tensile strength and toughness of spider silk make it a superior candidate for a wide range of medical and industrial applications. In this study, a 837 bp fragment which encode dragline silk gene （ASP） was cloned from the genome of spider （Araneus ventricosus） and subcloned into pGEX-6p-1 prokaryotic expression vector and pGFP-N2 eukaryotic expression vector, named pASG and pASN respectively. By induction at 16℃ for 24 h, the fusion protein GST-ASP was successfully expressed and characterized by Western blotting with anti-GST antibodies. The fusion proteins ASP-GFP was also successfully expressed in insect sf9 cells by the detection of bright green fluorescence. That indicated the ASP gene was corrected expressed in E. coli and eukaryotic cells, respectively. This study provides a beneficial attempt to exploiting the pathway of producting spider silk protein by genetic engineering.

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